advertisement
Browse Health
Dermatologist (skin)
7 years of experience
Accepting new patients

Credentials

Education ?

Medical School Score Rankings
Vanderbilt University (2004)
  •  
Top 50%

Awards & Distinctions ?

Associations
American Board of Dermatology
American Society for Dermatologic Surgery

Affiliations ?

Dr. Musiek is affiliated with 5 hospitals.

Hospital Affiliations

Score

Rankings

  • Pennsylvania Hospital University PA Health System
    800 Spruce St, Philadelphia, PA 19107
    •  
    Top 25%
  • Hospital of the University of PA
    3400 Spruce St, Philadelphia, PA 19104
    •  
    Top 25%
  • Barnes Jewish Hospital
    1 Barnes Jewish Hospital Plz, Saint Louis, MO 63110
    •  
    Top 25%
  • Missouri Baptist Hospital Sullivan
    751 Sappington Bridge Rd, Sullivan, MO 63080
    •  
    Top 50%
  • Philadelphia Veterans Affairs Medical Center
    3900 Woodland Ave, Philadelphia, PA 19104
  • Publications & Research

    Dr. Musiek has contributed to 1 publication.
    Title High-throughput Mitochondrial Genome Screening Method for Nonmelanoma Skin Cancer Using Multiplexed Temperature Gradient Capillary Electrophoresis.
    Date March 2005
    Journal Clinical Chemistry
    Excerpt

    BACKGROUND: We explored the utility of multiplexed temperature gradient capillary electrophoresis (TGCE) as a screening tool for identifying genetic changes in the human mitochondrial genome. We examined changes in mitochondrial DNA (mtDNA) in nonmelanoma skin cancers (NMSCs), using TGCE to resolve genetic differences contained within the tumors compared with the control DNA. METHODS: The entire mtDNA from NMSC tissue samples was amplified in 17 overlapping amplicons averaging 1.1 kb in size. Fourteen of these amplicons were digested with restriction endonucleases into as many as five smaller analyzable fragments. Digested tumor mtDNA amplicons were annealed with digested amplicons from the control DNA to form heteroduplexes in regions of DNA mismatch. TGCE was performed in a 96-well parallel format to detect mtDNA changes in a high-throughput fashion. RESULTS: TGCE resolved heteroduplexes from homoduplexes in singlet reactions and in multiplexed assays. Using a single programmed temperature gradient, we detected 18 of 20 mtDNA changes contained within the specimens. This system was also able to detect a single nucleotide change in a fragment as large as 2 kb. CONCLUSION: Multiplexed TGCE is a sensitive and high-throughput screening tool for identifying mtDNA variations.

    Similar doctors nearby

    Dr. Ilana Rosman

    Dermatology
    Saint Louis, MO

    Dr. Paul Klekotka

    Dermatology
    9 years experience
    Saint Louis, MO

    Dr. Milan Anadkat

    Dermatology
    10 years experience
    Saint Louis, MO

    Dr. Susan Bayliss

    Dermatology
    37 years experience
    Saint Louis, MO

    Dr. Andras Schaffer

    Dermatology - Dermatopathology
    Saint Louis, MO

    Dr. Anne Lind

    Pathology
    22 years experience
    Saint Louis, MO
    Search All Similar Doctors