Browse Health
Internist, Infectious Disease Specialist (virus, bacteria, parasites)
12 years of experience

Education ?

Medical School Score Rankings
Tufts University (1998)
  • Currently 3 of 4 apples
Top 50%

Awards & Distinctions ?

Associations
American Board of Internal Medicine

Affiliations ?

Dr. Clancy is affiliated with 3 hospitals.

Hospital Affilations

Score

Rankings

  • Providence Alaska Medical Center
    PO Box 196604, Anchorage, AK 99519
    • Currently 1 of 4 crosses
  • Alaska Regional Hospital
    2801 Debarr Rd, Anchorage, AK 99508
    • Currently 1 of 4 crosses
  • Providence Extended Care Center
    4900 Eagle St, Anchorage, AK 99503
  • Publications & Research

    Dr. Clancy has contributed to 63 publications.
    Title Immunosuppression is Essential for Successful Allogeneic Transplantation of the Metanephros.
    Date September 2009
    Journal Transplantation
    Excerpt

    Transplanted metanephroi vascularize and develop features of mature kidney. One group reported the intriguing finding that metanephric allografts and congenic, major histocompatibility complex-mismatched grafts developed without rejection in the absence of immunosuppression. Our experiments aim to investigate the hypothesis that metanephroi lack immunogenicity and identify immunosuppressives that do not inhibit development.

    Title Development and Functional Capacity of Transplanted Rat Metanephroi.
    Date April 2008
    Journal Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
    Excerpt

    Transplantation of embryonic kidneys (metanephroi) offers a potential solution to the problem of kidney donor shortage. The aim of this study was to characterise the haemodynamic capacity of transplanted rat metanephroi and to determine the number and maturity of the tubules.

    Title Single-center Experience of Encapsulating Peritoneal Sclerosis in Patients on Peritoneal Dialysis for End-stage Renal Failure.
    Date January 2006
    Journal Kidney International
    Excerpt

    Encapsulating peritoneal sclerosis (EPS) is a rare but serious complication in patients undergoing continuous ambulatory peritoneal dialysis (CAPD) or automated peritoneal dialysis (APD). It is characterized by a progressive, intra-abdominal, inflammatory process resulting in sheets of fibrous tissue that cover, bind, and constrict the viscera, thereby compromising the motility and function of the bowel. Although recent therapeutic approaches have been reported with variable success, the ability to detect reliably at an early stage patients at risk for EPS would be beneficial and allow treatment standardization. The aim of this study was to evaluate the clinical features of EPS and identify possible risk factors for its development in CAPD and APD patients.

    Title Widespread Emergence of Methicillin Resistance in Community-acquired Staphylococcus Aureus Infections in Denver.
    Date January 2006
    Journal Southern Medical Journal
    Excerpt

    BACKGROUND: Increasing rates of methicillin resistance among out-patient Staphylococcus aureus infections led us to assess the epidemiology and outcome of a local outbreak. METHODS: A retrospective cohort study of outpatient skin and soft tissue infections due to S aureus in 2003. RESULTS: From 2002 to mid-2004, the percentage of outpatient S aureus isolates resistant to methicillin increased from 6 to 45%. In multivariate analysis, only male sex and age greater than 18 years were associated with methicillin resistance. Methicillin resistance was common (>15%) among isolates from patients in nearly all subgroups evaluated. Pulsed field gel electrophoresis showed isolates related to USA 300, but methicillin-resistant strains had unusually high rates of quinolone resistance. CONCLUSIONS: A single strain of methicillin-resistant S aureus is responsible for the increase in skin infections in outpatients without traditional risk factors for infection with an antibiotic-resistant strain. In areas with high rates of methicillin-resistant S aureus outpatient infections, we recommend non-beta-lactam antibiotics for initial treatment of skin and soft tissue infections.

    Title Defining the Specialty.
    Date February 2005
    Journal Emergency Medicine Journal : Emj
    Title Clearing the Cervical Spine of Paediatric Trauma Patients.
    Date February 2005
    Journal Emergency Medicine Journal : Emj
    Excerpt

    To review the evidence available for clearance of the cervical spine in children under 16 years of age after trauma, and to provide guidance to enable this to be practised safely.

    Title Laryngopharyngeal Ph Measurement.
    Date February 2005
    Journal Emergency Medicine Journal : Emj
    Excerpt

    Most emergency department (ED) intubations are to prevent gastric contents aspiration. The incidence of aspiration is unknown and intubation has complications. Balancing these risks requires an idea of the incidence of aspiration. This study assessed one technique for investigating the aspiration risk in ED patients. Cricoid pressure is used to reduce this risk and the technique may also examine this manoeuvre.

    Title What is the Patient's Best Telephone Number?
    Date June 2004
    Journal Emergency Medicine Journal : Emj
    Title Overview of Research Designs.
    Date January 2003
    Journal Emergency Medicine Journal : Emj
    Title Induction of Sporulation in Saccharomyces Cerevisiae Leads to the Formation of N6-methyladenosine in Mrna: a Potential Mechanism for the Activity of the Ime4 Gene.
    Date November 2002
    Journal Nucleic Acids Research
    Excerpt

    N6-methyladenosine (m6A) is present at internal sites in mRNA isolated from all higher eukaryotes, but has not previously been detected in the mRNA of the yeast Saccharomyces cerevisiae. This nucleoside modification occurs only in a sequence- specific context that appears to be conserved across diverse species. The function of this modification is not fully established, but there is some indirect evidence that m6A may play a role in the efficiency of mRNA splicing, transport or translation. The S.cerevisiae gene IME4, which is important for induction of sporulation, is very similar to the human gene MT-A70, which has been shown to be a critical subunit of the human mRNA [N6-adenosine]-methyltransferase. This observation led to the hypothesis that yeast sporulation may be dependent upon methylation of yeast mRNA, mediated by Ime4p. In this study we show that induction of sporulation leads to the appearance of low levels of m6A in yeast mRNA and that this modification requires IME4. Moreover, single amino acid substitutions in the putative catalytic residues of Ime4p lead to severe sporulation defects in a strain whose sporulation ability is completely dependent on this protein. Collectively, these data suggest very strongly that the activation of sporulation by Ime4p is the result of its proposed methyltransferase activity and provide the most direct evidence to date of a physiologic role of m6A in a gene regulatory pathway.

    Title A New Way of Demonstrating How Close the Evidence is to the Question You Asked.
    Date December 2001
    Journal Emergency Medicine Journal : Emj
    Title Tube Migration Causing Gastric Outlet Obstruction: an Unusual Complication of Percutaneous Endoscopic Gastrostomy.
    Date February 2001
    Journal Endoscopy
    Title Bronchoscopic Removal of an Inhaled, Sharp, Foreign Body: an Unusual Complication.
    Date February 2000
    Journal The Journal of Laryngology and Otology
    Excerpt

    A case is reported in which an inhaled sewing needle, stuck fast in the trachea, became displaced through the tracheal wall during attempted removal via flexible bronchoscopy. The inherent risks and pitfalls of this procedure are highlighted.

    Title How to Conduct an Appraisal of a Senior House Officer.
    Date October 1999
    Journal Journal of Accident & Emergency Medicine
    Title Should Uk Emergency Physicians Undertake Diagnostic Ultrasound Examinations?
    Date October 1999
    Journal Journal of Accident & Emergency Medicine
    Excerpt

    From the published evidence there is no doubt that emergency physicians in America can undertake focused ultrasound examinations and that, by extrapolation, this would also be the case for UK emergency physicians. If this skill is to become part of the diagnostic armamentarium of the emergency physician, however, it needs to be demonstrated to be cost effective compared with the alternatives already available to the hospital. Trials to test for this benefit should adopt a hospital and not an emergency department perspective if the results are to influence health policy and specialty training.

    Title Persistent "haematoma".
    Date October 1999
    Journal Journal of Accident & Emergency Medicine
    Title Clearing the Cervical Spine of Adult Victims of Trauma.
    Date July 1999
    Journal Journal of Accident & Emergency Medicine
    Title Use of the Cochrane Library by Emergency Physicians.
    Date July 1999
    Journal Journal of Accident & Emergency Medicine
    Title Point of Care Testing in the Accident and Emergency Department: a Cost Analysis and Exploration of Financial Incentives to Use the Technology Within the Hospital.
    Date March 1999
    Journal Journal of Health Services Research & Policy
    Excerpt

    To compare the costs of current arrangements for testing emergency blood samples from patients attending an accident and emergency (A&E) department in a large teaching hospital in England with point of care testing (POCT).

    Title Meiosis: Step-by-step Through Sporulation.
    Date September 1998
    Journal Current Biology : Cb
    Excerpt

    A transcription factor, Ndt80p, has been identified that has a critical role in the pathway that controls meiosis--sporulation--in budding yeast. Ndt80p coordinately controls genes that mediate spore formation and progression through the two meiotic divisions; it may also be a target of a checkpoint control.

    Title Research in Accident and Emergency Medicine.
    Date April 1997
    Journal Journal of Accident & Emergency Medicine
    Title Changes in Stroke Distance in Response to Haemorrhage in a Swine Model.
    Date February 1997
    Journal Journal of Accident & Emergency Medicine
    Excerpt

    To determine, using an animal model of blood loss, (1) if stroke distance, derived non-invasively from the time integral of the maximum velocity of red cells in the aorta, changed to a greater extent than heart rate and mean arterial pressure (MAP), which are recognised to be unreliable indicators of blood loss; (2) if changes in stroke distance reflected changes in stroke volume derived from thermodilution cardiac output measurements.

    Title The S. Cerevisiae Nitrogen Starvation-induced Yvh1p and Ptp2p Phosphatases Play a Role in Control of Sporulation.
    Date January 1997
    Journal Yeast (chichester, England)
    Excerpt

    Starvation for nitrogen in the absence of a fermentable carbon source causes diploid Saccharomyces cerevisiae cells to leave vegetative growth, enter meiosis, and sporulare; the former nutritional condition also induces expression of the YVH1 gene that encodes a protein phosphatase. This correlation prompted us to determine whether the Yvh1p phosphatase was a participant in the network that controls the onset of meiosis and sporulation. We found that expression of the IME2 gene, encoding a protein kinase homologue required for meiosis- and sporulation-specific gene expression, is decreased in a yvh1 disrupted strain. We also observed a decrease, albeit a smaller one, in the expression of IME1 which encodes an activator protein required for IME2 expression. Under identical experimental conditions, expression of the MCKI and IME4 genes (which promote sporulation but do not require Ime1p for expression) was not affected. These results demonstrate the specificity of the yvh1 disruption phenotype. They suggest that decreased steady-state levels of IME1 and IME2 mRNA were not merely the result of non-specific adverse affects on nucleic acid metabolism caused by the yvh1 disruption. Sporulation of a homozygous yvh1 disruption mutant was delayed and less efficient overall compared to an isogenic wild-type strain, a result which correlates with decreased IME1 and IME2 gene expression. We also observed that expression of the PTP2 tyrosine phosphatase gene (a negative regulator of the osmosensing MAP kinase cascade), but not the PTP1 gene (also encoding a tyrosine phosphatase) was induced by nitrogen-starvation. Although disruption of PTP2 alone did not demonstrably affect sporulation or IME2 gene expression, sporulation was decreased more in a yvh1, ptp2 double mutant than in a yvh1 single mutant; it was nearly abolished in the double mutant. These data suggest that the YVH1 and PTP2 encoded phosphatases likely participate in the control network regulating meiosis and sporulation. Expression of YVH1 and PTP2 was not affected by nitrogen source quality (asparagine compared to proline) suggesting that nitrogen starvation-induced YVH1 and PTP2 expression and sensitivity to nitrogen catabolite repression are on two different branches of the nitrogen regulatory network.

    Title Volumetric Flow Estimation in Vivo and in Vitro Using Pulsed-doppler Ultrasound.
    Date January 1997
    Journal Ultrasound in Medicine & Biology
    Excerpt

    The measurement of volumetric blood flow in small vessels in vitro and in vivo poses a significant technological challenge. In this study, two pulsatile flow models were developed, one with a 3.2-mm lumen diameter and one with a 12.7-mm lumen diameter, to assess the accuracy of volumetric flow estimation of two pulsed-Doppler devices, a Crystal Biotech VF1 20-MHz system with either a cuff-mounted or a needle-mounted probe and an Advanced Technology Laboratories Ultramark 9 High Definition Imaging system with a 5-MHz linear array transducer. The VF1 volumetric flow error was measured in the 3.2-mm phantom over a variety of pulsatile and continuous waveforms. The accuracy of the VF1 was also tested in porcine femoral and renal arteries. VF1 volumetric flow error ranged from 4.8% to 54.3% in the in vivo studies. The ATL demonstrated similar volumetric flow errors in the porcine femoral artery (approximately 3.2 mm diameter), but these errors were reduced to < or = 17.4% in the 12.7-mm-diameter in vitro flow model.

    Title Benefits of Immediate Printing of Blood Test Results Within an A&e Department.
    Date July 1996
    Journal Journal of Accident & Emergency Medicine
    Title The Use of Ultrasound in the Non-invasive Detection of Changes in the Renal Circulation in Response to Blood Loss Using an Animal Model.
    Date February 1996
    Journal Resuscitation
    Excerpt

    Using a continuous haemorrhage model, 8 anaesthetised swine were bled 1 ml/kg per min for 30 min. The resistance index (RI) of the main renal artery, interlobar and arcuate vessels all significantly increased. Cortical Doppler signals were lost in 4 animals at a mean arterial pressure of 26 mmHg. After reinfusion of blood and normal saline only the RI of the interlobar vessels was significantly different from baseline readings. Ultrasound demonstrated non-invasively changes in regional blood flow within the kidney in response to hypovolaemic shock.

    Title Pneumatic Anti-shock Garment--does It Have a Future?
    Date December 1995
    Journal Journal of Accident & Emergency Medicine
    Excerpt

    A total of 100 accident and emergency (A&E) departments in the UK responded to a questionnaire about their use of the pneumatic anti-shock garment (PASG). Less than one in 10 departments used PASG in their prehospital care system, less than one in five departments used PASG during in-patient care, and there was wide variation in PASG usage in those situations for which their use is recommended by the Advanced Trauma Life Support (ATLS) course.

    Title The Spr3 Gene Encodes a Sporulation-specific Homologue of the Yeast Cdc3/10/11/12 Family of Bud Neck Microfilaments and is Regulated by Abfi.
    Date December 1995
    Journal Gene
    Excerpt

    The SPR3 gene is selectively activated only during the sporulation phase of the Saccharomyces cerevisiae (Sc) life cycle. The predicted amino acid (aa) sequence has homology to microfilament proteins that are involved in cytokinesis and other proteins of unknown function. These include the products of Sc cell division cycle (CDC) genes involved in bud formation (Cdc3p, Cdc10p, Cdc11p and Cdc12p), Candida albicans proteins that accumulate in the hyphal phase (CaCdc3p and CaCdc10p), mouse brain-specific (H5p) and lymphocyte (Diff6p) proteins, Drosophila melanogaster (Dm) protein Pnutp (which is localized to the cleavage furrow of dividing cells), a Diff6p homologue (DmDiff6p), and the Sc septin protein (Sep1hp), a homologue of the 10-nm filament proteins of Sc. One strongly conserved region contains a potential ATP-GTP-binding domain. Primer extension analysis revealed six major transcription start points (tsp) beginning at -142 relative to the ATG start codon. The sequence immediately upstream from the tsp contains consensus binding sites for the HAP2/3/4 and ABFI transcription factors, a T-rich sequence and two putative novel elements for mid to late sporulation, termed SPR3 and PAL. Electrophoretic mobility shift assay (EMSA) and footprint analyses demonstrated that the ABFI protein binds to a region containing the putative ABFI site in vitro, and site-directed mutagenesis showed that the ABFI motif is essential for expression of SPR3 at the appropriate stage in sporulating cells.

    Title Nosocomial Infection Due to Enterococci Attributed to a Fluidized Microsphere Bed.
    Date April 1995
    Journal The Journal of Hospital Infection
    Title The Effect of Audit and Feedback on Data Recording in the Accident and Emergency Department.
    Date November 1994
    Journal Journal of Accident & Emergency Medicine
    Excerpt

    Thirteen newly appointed senior house officers (SHOs) at two hospitals were assessed in the recording of two variables: Glasgow Coma Scale (GCS) and diagnostic coding at Hospital A, and GCS at Hospital B. At Hospitals A and B baseline recordings of these variables were established. Active feedback (in the form of data presentation and discussion) was given at 6 and 11 weeks and a final 4-week audit was performed on GCS recordings at 20 weeks. Analysis of GCS recordings of head injuries at Hospitals A and B showed an increase from baseline values of 162/401 (40%) to 336/420 (80%) after feedback 1, and after feedback 2 recordings increased to 379/429 (88%). This was maintained in the last 4-week audit which showed recordings of 220/244 (90%). Further analysis of Hospital A's data showed the SHOs recorded diagnostic coding in 1335/4406 (30%) of cases. After feedback 1, recordings increased to 2550/3327 (77%). After feedback 2 there was no change in recording 2712/3530 (77%). Active feedback improves data recording. The first feedback has a greater impact than the second. Two feedbacks produce a sustained effect in 6 months.

    Title Nosocomial Infection and Microsphere Beds.
    Date September 1993
    Journal Lancet
    Title Undergraduate Medical Student Education: the Case for a National Curriculum.
    Date April 1993
    Journal Archives of Emergency Medicine
    Excerpt

    Thirty-eight teaching hospital affiliated accident and emergency departments were surveyed by post to try to find if there had been any change in undergraduate medical student teaching over the past 10 years. Twenty-six departments replied. The results showed that although there has been an improvement in the teaching of A&E medicine to undergraduates in the past few years there are still some medical schools where an A&E attachment is not mandatory. In those departments providing teaching, there is wide variation in course duration and content.

    Title Now Where Did I Put That Paper?: A Simple Reference System for Accident and Emergency Medicine Related Papers and Publications.
    Date April 1993
    Journal Archives of Emergency Medicine
    Title Carpometacarpal Dislocation: an Unusual Complex Injury of the Hand.
    Date May 1992
    Journal Archives of Emergency Medicine
    Title Ime4, a Gene That Mediates Mat and Nutritional Control of Meiosis in Saccharomyces Cerevisiae.
    Date April 1992
    Journal Molecular and Cellular Biology
    Excerpt

    In the yeast Saccharomyces cerevisiae, sporulation occurs in response to nutritional and genetic signals. The process is initiated when nutrient availability limits mitotic growth, but only in MATa/MAT alpha diploid cells. Under these conditions, the cells express an activator of meiosis (IME1), which is required for the expression of early sporulation-specific genes. We describe a new gene, IME4, whose activity is essential for IME1 transcript accumulation and sporulation. The IME4 transcript was induced in starved MATa/MAT alpha diploids but not in other cell types. In addition, excess IME4 promoted sporulation in mat-insufficient cells. Thus, IME4 appears to activate IME1 in response to cell type and nutritional signals. We have also explored the interactions between IME4 and two genes that are known to regulate IME1 expression. Normally, cells that lack complete MAT information cannot sporulate; when such strains lack RME1 activity or contain the semidominant RES1-1 mutation, however, they can express IME1 and sporulate to low levels. Our results show that mat-insufficient strains containing rme1::LEU2 or RES1-1 bypass mutations still retain MAT control of IME4 expression. Even though IME4 levels remained low, the rme1::LEU2 and RES1-1 mutations allowed IME1 accumulation, implying that these mutations do not require IME4 to exert their effects. In accord with this interpretation, the RES1-1 mutation allowed IME1 accumulation in MATa/MAT alpha strains that contain ime4::LEU2 alleles. These strains still sporulated poorly, suggesting that IME4 plays a role in sporulation in addition to promoting IME1 transcript accumulation. IME4 is located between ADE5 and LYS5 on chromosome VII.

    Title The Respiratory Rate.
    Date December 1991
    Journal Archives of Emergency Medicine
    Title Use of Retrograde Urethrography in the Resuscitation Room.
    Date December 1991
    Journal Archives of Emergency Medicine
    Title A Study of the Eye Care Provided by an Accident and Emergency Department.
    Date October 1991
    Journal Archives of Emergency Medicine
    Excerpt

    The eye care provided by an Accident and Emergency (A&E) Department was prospectively studied over a 2-month period. Six out of a total of 506 patients were deemed to have been treated unsatisfactorily by the A&E Department. None of these patients suffered serious sequelae. Approximately one in three of those patients seen solely by A&E staff failed to have their visual acuity recorded.

    Title An Rme1-independent Pathway for Sporulation Control in Saccharomyces Cerevisiae Acts Through Ime1 Transcript Accumulation.
    Date April 1991
    Journal Genetics
    Excerpt

    The RES1-1 mutation was isolated on the basis of its ability to allow MATa/MAT alpha diploid Saccharomyces cerevisiae cells to express a late sporulation-regulated gene, SPR3, in the presence of excess copies of RME1. RME1 is a repressor of meiosis that is normally expressed in cells that lack the a1/alpha 2 repressor encoded by MAT. The RES1-1 mutation also supports sporulation in mat-insufficient diploids. This phenotype does not result from a failure to express RME1 and is not due to activation of the silent copies of mating type information. RES1-1 activates sporulation by allowing IME1 accumulation in all cell types, irrespective of the presence of the MAT products. IME1 is still responsive to RME1 in RES1-1 cells, since double mutants (rme1 RES1-1) that are deficient at MAT can sporulate better than either single mutant. RES1-1 is not an allele of IME1.

    Title Functional Analysis of the Sporulation-specific Spr6 Gene of Saccharomyces Cerevisiae.
    Date January 1991
    Journal Current Genetics
    Excerpt

    The SPR6 gene of Saccharomyces cerevisiae encodes a moderately abundant RNA that is present at high levels only during sporulation. The gene contains a long open reading frame that could encode a hydrophilic protein approximately 21 kDa in size. This protein is probably produced by the yeast, because the lacZ gene of Escherichia coli is expressed during sporulation when fused to SPR6 in the expected reading frame. SPR6 is inessential for sporulation; mutants that lack SPR6 activity sporulate normally and produce viable ascospores. Nonetheless, the SPR6 gene encodes a function that is relevant to sporulating cells; the wild-type allele can enhance sporulation in strains that are defective for several SPR functions. SPR6 is located on chromosome V, 14.4 centimorgans centromere-distal to MET6.

    Title Differential Regulation of Sta Genes of Saccharomyces Cerevisiae.
    Date December 1990
    Journal Molecular & General Genetics : Mgg
    Excerpt

    The single glucoamylase gene (SGA1) of the yeast Saccharomyces cerevisiae is expressed exclusively during the sporulation phase of the life cycle. Enzymatic studies and nucleic acid sequence comparisons have shown that the SGA1 glucoamylase is closely related to the secreted enzymes of S. cerevisiae var. diastaticus. The latter are encoded by any of three unlinked STA genes, which have been proposed to derive from the ancestral SGA1 form by genomic rearrangement. We show that the regulation of SGA1 is distinct from that of the other members of the STA gene family. SGA1 expression did not respond to STA10, the primary determinant of glucoamylase expression from STA2. Unlike STA2, SGA1 was not regulated directly by the mating type locus. Expression of SGA1 depended on the function of the MAT products in supporting sporulation and not on the formation of haploid progeny spores or on the composition of the mating type locus per se. We conclude that the STA genes acquired regulation by STA10 and MAT by the genomic rearrangements that led to their formation. This regulation is thus distinct from that of the ancestral SGA1 gene.

    Title The Erroneous Use by an Accident and Emergency Department of Plain Abdominal Radiographs in the Diagnosis of Constipation.
    Date March 1990
    Journal Archives of Emergency Medicine
    Title Answer Please. Blount's Disease: Tibia Vara.
    Date January 1990
    Journal Orthopedics
    Excerpt

    Infantile tibia vara, although uncommon compared to normal physiologic bowing, is significant due to the resultant progressive deformities if not diagnosed early. The key radiographic finding is medial tibial metaphyseal beaking and fragmentation. Although the etiology is unclear, it appears to be an acquired growth disturbance of the proximal medial tibial epiphysis, ossification center, and metaphysis rather than any type of avascular necrosis. The Langenskiƶld six-stage classification is useful for the diagnostic, prognostic, and treatment staging of the disease. The adolescent form is less common and less severe with only slight irregular thickening of the physis present on radiograph. The MDA appears to be the most reproducible method for measuring angular deformities in both variants of the disease. Treatment of the infantile stages I or II consists of observation and bracing. Stage III or IV disease in children over age 3 years with more than 5 degrees of varus angulation should be treated with corrective valgus osteotomy. Stages V or VI may require repeated surgical treatment with multiple osteotomies and possible contralateral epiphysiodesis. The adolescent form frequently requires surgery when there is significant physical impairment related to the severe genu varum.

    Title Total Hip Replacement and Antithrombotic Prophylaxis.
    Date December 1989
    Journal British Journal of Hospital Medicine
    Excerpt

    British orthopaedic surgeons were surveyed to assess the practice of prophylaxis against thromboembolism in patients undergoing total hip replacements. Of 690 surgeons, 348 (50.4%) do not routinely use pharmacological prophylaxis (190 use no prophylaxis while 158 use a mechanical form of prophylaxis), 289 (41.9%) surgeons use a single pharmacological agent (with or without a mechanical form of prophylaxis), and the remaining 53 (7.7%) use two or more pharmacological agents.

    Title The Diagnosis of Ectopic Pregnancy in an Accident and Emergency Department.
    Date November 1989
    Journal Archives of Emergency Medicine
    Excerpt

    The correct diagnosis was initially suspected in 32 (53%) of 60 patients with ectopic pregnancy who attended an accident and emergency department. Incorrect diagnoses were made because ectopic pregnancy was not considered or because relevant symptoms and signs were missed or misinterpreted. Three patients had been 'sterilized'. Twenty-four patients (40%) had abdominal pain or vaginal bleeding for more than 1 week before attending. Fever and leucocytosis were wrongly attributed to pelvic infection. Pregnancy tests were positive in 56% of the patients tested.

    Title A Stand for a Defibrillator.
    Date September 1989
    Journal Anaesthesia
    Title A Study of the Performance of Cricothyroidotomy on Cadavers Using the Minitrach Ii.
    Date August 1989
    Journal Archives of Emergency Medicine
    Excerpt

    Cricothyroidotomy was attempted on 15 cadavers. Five out of 15 doctors failed to cannulate the trachea. The high failure rate and incidence of complications are discussed. All doctors found the experience beneficial.

    Title Treatment Options in Simple Unicameral Bone Cysts.
    Date August 1989
    Journal Orthopedics
    Title False Aneurysm of the Axillary Artery As a Complication of the Modified Bristow Procedure.
    Date June 1989
    Journal Injury
    Title Dependence of Inessential Late Gene Expression on Early Meiotic Events in Saccharomyces Cerevisiae.
    Date May 1989
    Journal Molecular & General Genetics : Mgg
    Excerpt

    SPR3 is one of at least nine genes which are expressed in sporulating Saccharomyces cerevisiae cells at the time of meiosis I. We show below that strains homozygous for null alleles of SPR3 are capable of normal meiosis and the production of viable ascospores. We have also monitored SPR3 expression in a series of strains that are defective in meiotic development, using an SPR3:lacZ fusion carried on a single copy plasmid. beta-Galactosidase activity occurred at wild-type levels in diploid strains homozygous for mutations in spo13, rad50, rad57 and cdc9, but was greatly reduced in strains carrying cdc8 or spo7 defects. We conclude that SPR3 expression is a valid monitor of early meiotic development, even though the gene is inessential for the sporulation process.

    Title Characterization and Localization of the Sporulation Glucoamylase of Saccharomyces Cerevisiae.
    Date April 1989
    Journal Biochimica Et Biophysica Acta
    Excerpt

    Glucoamylase (SGA) was purified approximately 250-fold from sporulating Saccharomyces cerevisiae cells. The partially purified enzyme was active against glycogen, starch, maltotriose and maltose. It exhibited maximum catalytic activity against glycogen at pH 5.5. The enzyme appears to be glycosylated, because it bound to lentil-lectin Sepharose. SGA was expressed in vegetatively growing cells under the control of the GAL1 promoter, and the cellular location of the enzymatic activity determined by fractionation techniques. SGA was preferentially recovered in fractions which were enriched for the vacuolar hydrolases, carboxypeptidase Y and alpha-mannosidase.

    Title Sporulation-regulated Genes of Saccharomyces Cerevisiae.
    Date October 1988
    Journal Current Genetics
    Excerpt

    We have characterized 46 hybrid phage which hybridize preferentially to mRNA from sporulating cells. Cross-hybridization experiments demonstrate that 27 distinct SPR (Sporulation regulated) sequences are represented among these phage. The SPR genes can be grouped into three classes: early, middle, and late. The early class shows an accumulation of transcripts soon after transfer to sporulation medium and continues to accumulate RNA throughout sporulation. Transcripts of the middle class increase in level at about the time of DNA synthesis, rise rapidly in abundance until meiosis II, then accumulate more slowly for at least the next 3 h. Late gene transcripts begin to accumulate at about the time of meiosis I, increase 10- to 20-fold in the next 2 h, then remain constant in late sporulating cells.

    Title Transcriptional Regulation of Sporulation Genes in Yeast.
    Date February 1988
    Journal Molecular & General Genetics : Mgg
    Excerpt

    The relative transcription rates of three sporulation-regulated genes of yeast (SPR1, SPR2 and SPR3) were determined at intervals during sporulation, using a filter binding assay. The binding of in vivo labeled RNA to the corresponding DNAs increased 3- to 12-fold at the time of meiosis I, in parallel with the accumulation of the SPR transcripts. SPR1 and SPR3 mRNA abundance increased from less than 0.7 to 130 and 90 copies per cell, respectively, between the time of shift to sporulation medium and the initiation of spore formation. This represented a 150-to 200-fold increase in the steady-state levels of these RNAs. Similarly, the levels of beta-galactosidase present in sporulating cells harboring fusions between SPR3 and Escherichia coli lacZ increased at least 700-fold. We conclude that SPR1, SPR2 and SPR3 transcription is modulated during sporulation, possibly in response to earlier events in the process.

    Title The Formulation of Buffers and Media for Enzyme Histochemistry.
    Date July 1987
    Journal The Histochemical Journal
    Excerpt

    Buffer solutions and incubating media for enzyme histochemistry are discussed in terms of pH, ionic strength and buffering capacity. A specially written program is presented. This program enables (i) buffers and media of known pH and ionic strength to be formulated; (ii) the ionic strength of a buffer solution of known molarity, and (iii) the thermodynamic acid dissociation constant of a buffer substance, to be calculated.

    Title The Effects of Anabolic Agents and Breed on the Fibers of the Longissimus Muscle of Male Cattle.
    Date September 1986
    Journal Journal of Animal Science
    Excerpt

    Sample of longissimus muscle were taken from carcasses of steers, steers implanted with anabolic agents and bulls of Friesian and Charolais X Friesian breeds of cattle. Percent and mean cross-sectional areas (CSA) of three myofiber types (beta R, alpha R and alpha W) were determined. The percentage of beta R myofibers did not vary significantly with treatment. The implanted steers had 26% more alpha R and 8% less alpha W myofibers than the untreated steers, while the bulls had 33% more alpha R and 20% less alpha W myofibers than the implanted steers (P less than .001). In the implanted steers the mean CSA of the beta R myofibers was significantly greater than that of the untreated steers, but did not differ from that of the bull. The mean CSA of the alpha R myofibers increased considerably with treatment, but only that of the bull was significantly greater than that of the untreated steers. The mean CSA of the alpha W myofibers in the implanted steers was identical with that of the untreated steers and significantly smaller than that of the bulls. In comparison to the untreated steers, significant hypertrophy of all three myofiber types occurred in bulls. These findings demonstrate a significant increase in the oxidative capacity of the longissimus when the levels of both endogenous and exogenous anabolic agents are increased. They are also consistent with the greater efficiency of deposition of protein obtained with implanted steers and bulls.(ABSTRACT TRUNCATED AT 250 WORDS)

    Title Isolation of Genes Expressed Preferentially During Sporulation in the Yeast Saccharomyces Cerevisiae.
    Date July 1983
    Journal Proceedings of the National Academy of Sciences of the United States of America
    Excerpt

    A library of Saccharomyces cerevisiae DNA in the vector lambda Charon 28 was probed for sequences complementary to cDNA made from poly(A)+ RNA isolated from the well-sporulating yeast strain AP1 a/alpha. The RNA was isolated from cells that had been incubated 7, 9, 11, and 13 hr in sporulation medium. DNA complementary to poly(A)+ RNA from alpha/alpha(nonsporulating) AP1 was used as a control, and 46 bacteriophage that gave a stronger response with a/alpha cDNA than with alpha/alpha cDNA were obtained in a screening of three yeast genomes worth of DNA. Two of the bacteriophage appeared to contain a/alpha-specific genes, in that they hybridized to cDNA from vegetative a/alpha RNA. The rest appeared to correspond to a/alpha genes expressed preferentially during sporulation. Restriction endonuclease analysis of four of the cloned sequences revealed a single major region of transcription in each; these regions ranged in size from 2.5 to 4.0 kilobases. RNA blot analysis showed that, in three of the four cases, transcripts of two different sizes were homologous to the cloned sequence. In all four cases, the homologous transcripts appeared at about 7 hr and were decreasing in amount by 13 hr. These results provide evidence for transcriptional control of genes expressed during sporulation and for at least one group of genes that is turned on at about the time of meiosis I in sporulation.

    Title Developmental Regulation of a Sporulation-specific Enzyme Activity in Saccharomyces Cerevisiae.
    Date October 1982
    Journal Molecular and Cellular Biology
    Excerpt

    An alpha-glucosidase activity (SAG) occurs in a/alpha Saccharomyces cerevisiae cells beginning at about 8 to 10 h after the initiation of sporulation. This enzyme is responsible for the rapid degradation of intracellular glycogen which follows the completion of meiosis in these cells. SAG differs from similar activities present in vegetative cells and appears to be a sporulation-specific enzyme. Cells arrested at various stages in sporulation (DNA replication, recombination, meiosis I, and meiosis II) were examined for SAG activity; the results show that SAG appearance depends on DNA synthesis and some recombination events but not on the meiotic divisions.

    Title Localization of Proteins in the Inner and Outer Membranes of Caulobacter Crescentus.
    Date August 1982
    Journal Biochimica Et Biophysica Acta
    Excerpt

    Cytoplasmic and outer membranes of Caulobacter crescentus were separated by isopycnic sucrose gradient centrifugation into two peaks with buoyant densities 1.22 and 1.14 g/cm3. These peaks were identified as outer and cytoplasmic membranes by the enrichment of malate dehydrogenase and NADH oxidase in the lower density peak and the presence of flagellin, a cell surface protein, in the heavier peak. The identity of the heavier peak as outer membrane was confirmed by labeling of cells with diazotized [35S]sulfanilic acid, a reagent that does not penetrate intact cells. Under these conditions only outer membrane proteins were substituted by the sulfanilic acid. The distribution of proteins between the cytoplasmic and outer membranes were examined by the analysis of [35S]methionine-labeled membranes by SDS-polyacrylamide and two-dimensional gel electrophoresis. These results showed that the inner and outer membranes contain approximately equal numbers of proteins, and that the distribution of these proteins between the two layers is highly asymmetric. Although many of the proteins could be assigned to one or the other membrane fraction, a number of the outer membrane proteins in the 32 000-100 000 molecular weight range frequently contaminate the inner membrane fractions. The implications of these results for membrane isolation and separation in C. crescentus are discussed.

    Title Properties of Polyadenylate-associated Ribonucleic Acid from Saccharomyces Cerevisiae Ascospores.
    Date May 1981
    Journal Journal of Bacteriology
    Excerpt

    Bulk ribonucleic acid (RNA) was isolated from mechanically disrupted ascospores of Saccharomyces cerevisiae. After two passes over an oligo (dT10) cellulose column, the portion which bound, called poly(A)(+), was characterized. It is heterodisperse in size with a mean molecular weight of approximately 4 X 10(5), but contains some species as large as 7 X 10(5). The base composition is similar to vegetative poly(A)(+) RNA. The polyadenylate segment is also heterogenous in size, ranging from 90 to 20 bases in length, with a peak at approximately 60 nucleotides in length. Pulse-labeling of asci with [3H-methyl]methionine yields two "caps," 7-methyl guanosine-5'-triphosphoryl-5'-adenosine (or guanosine) identical to that found in vegetative poly(A)(+) RNA. The poly(A)(+) RNA in spores is found in polyribosomes which are, on the average, smaller than vegetative ones. Long-term labeling studies indicate that the fraction of poly(A)(+) RNA in spores is similar to that in vegetative cells.

    Title The Assay of Alpha-amylase.
    Date January 1970
    Journal The Biochemical Journal
    Title The Inhibition of Wheat Beta-amylase.
    Date February 1969
    Journal The Biochemical Journal
    Title Enzymic Polymerization of Monosaccharides. I. The Enzymic Polymerization of D-galactose.
    Date December 1967
    Journal Archives of Biochemistry and Biophysics
    Title Enzymic Polymerization of Monosaccharides. Ii. Synthesis of 2-acetamido-2-deoxy-6-o-(alpha-d-galactopyranosyl)-d-glucose (n-acetylmelibiosamine).
    Date December 1967
    Journal Archives of Biochemistry and Biophysics

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