Browse Health
Ophthalmologist (eyes)
4 years of experience
Accepting new patients

Education ?

Medical School Score
Texas Tech University (2006)
  • Currently 2 of 4 apples

Affiliations ?

Dr. Allen is affiliated with 8 hospitals.

Hospital Affilations

Score

Rankings

  • Texas Health Presbyterian Hospital Plano
    6200 W Parker Rd, Plano, TX 75093
    • Currently 4 of 4 crosses
    Top 25%
  • Children's Medical Center of Dallas
    1935 Motor St, Dallas, TX 75235
    • Currently 4 of 4 crosses
    Top 25%
  • Texas Health Presbyterian Hospital Of Dallas
    8200 Walnut Hill Ln, Dallas, TX 75231
    • Currently 3 of 4 crosses
    Top 50%
  • Texas Institute For Surgery At Presbyterian Hospit
    7115 Greenville Ave, Dallas, TX 75231
  • Presbyterian Plano Center For Diagnostics & Surger
    6020 W Parker Rd, Plano, TX 75093
  • Texas Health Dallas
  • Texas Health Plano
  • Harris Methodist - Springwood
    1608 Hospital Pkwy, Bedford, TX 76022
  • Publications & Research

    Dr. Allen has contributed to 111 publications.
    Title Risk Factors for Intraocular Pressure Elevation After Descemet Stripping Automated Endothelial Keratoplasty.
    Date October 2010
    Journal Eye & Contact Lens
    Excerpt

    To identify the incidence of and risk factors for intraocular pressure (IOP) elevation after Descemet stripping automated endothelial keratoplasty (DSAEK).

    Title Marine Microorganisms.
    Date July 2010
    Journal Science (new York, N.y.)
    Title Frequency of Nocturnal Hypoxia in Clinically Stable Patients During Stroke Rehabilitation.
    Date June 2010
    Journal Clinical Rehabilitation
    Excerpt

    Sleep disordered breathing is common in patients with cerebrovascular disease. Nocturnal hypoxia may lead to daytime tiredness and cognitive impairment, thus affecting progress. This study assessed the prevalence of nocturnal hypoxia during rehabilitation from stroke.

    Title Wus and Stm-based Reporter Genes for Studying Meristem Development in Poplar.
    Date June 2009
    Journal Plant Cell Reports
    Excerpt

    We describe the development of a reporter system for monitoring meristem initiation in poplar using promoters of poplar homologs to the meristem-active regulatory genes WUSCHEL (WUS) and SHOOTMERISTEMLESS (STM). When ~3 kb of the 5' flanking regions of close homologs were used to drive expression of the GUSPlus gene, 50-60% of the transgenic events showed expression in apical and axillary meristems. However, expression was also common in other organs, including in leaf veins (40 and 46% of WUS and STM transgenic events, respectively) and hydathodes (56% of WUS transgenic events). Histochemical GUS staining of explants during callogenesis and shoot regeneration using in vitro stems as explants showed that expression was detectable prior to visible shoot development, starting 3-15 days after explants were placed onto callus inducing medium. A minority of WUS and STM events also showed expression in the cambium, phloem, or xylem of regenerated, greenhouse grown plants undergoing secondary growth. Based on microarray gene expression data, a paralog of poplar WUS was detectably up-regulated during shoot initiation, but the other paralog was not. Both paralogs of poplar STM were down-regulated threefold to sixfold during early callus initiation. We identified 15-35 copies of cytokinin response regulator binding motifs (ARR1AT) and one copy of the auxin response element (AuxRE) in both promoters. Several of the events recovered may be useful for studying the process of primary and secondary meristem development, including treatments intended to stimulate meristem development to promote clonal propagation and genetic transformation.

    Title Enhanced Allergen-induced Airway Inflammation in Paucity of Lymph Node T Cell (plt) Mutant Mice.
    Date February 2007
    Journal The Journal of Allergy and Clinical Immunology
    Excerpt

    BACKGROUND: Dendritic cells and lymphocytes play a central role in allergic asthma. Chemokines for these cells include the CCR7 agonists secondary lymphoid chemokine/CCL21 and EBV-induced lymphoid chemokine/CCL19, but their role in allergic asthma is poorly understood. OBJECTIVE: We sought to determine the effects of abrogation of lymphoid tissue expression of CCR7 agonists on allergic airway responses. METHODS: Paucity of lymphocyte T cell (plt) mutant mice, deficient in EBV-induced lymphoid chemokine/CCL19 and the lymphoid form of secondary lymphoid chemokine/CCL21, were evaluated in an established ovalbumin (OVA)-induced asthma model (plt-OVA group) and compared with similarly immunized +/+ BALB/c mice (+/+OVA group). RESULTS: APTI responses to methacholine increased similarly in OVA-challenged plt and +/+ mice. However, airway inflammation was strikingly enhanced in plt-OVA mutants over +/+OVA mice and included increased numbers of eosinophils, CD4 and B cells, neutrophils, and total leukocytes in bronchoalveolar lavage fluid and inflammatory cell cuffing around pulmonary arterioles. Enhanced airway inflammation was accompanied by an increase in lung T(H)2 activity, with increased levels of IL-4 and monocyte-derived chemoattractant/CCL22. CONCLUSIONS: Induction of allergic asthma in mutant mice with impaired CCR7 responses results in characteristics that resemble severe asthma in human subjects, including severe bronchial lymphocytosis, eosinophilia, and neutrophilia, but not in enhancement in airway hyperreactivity. CLINICAL IMPLICATIONS: Disruption of chemokines responsible for trafficking of antigen-processing cells and lymphocytes to the draining lymph nodes might lead to enhanced allergic airway responses.

    Title Photosynthesis by Isolated Chloroplasts Ix. Photosynthetic Phosphorylation and Co(2) Assimilation in Different Species.
    Date May 2006
    Journal Plant Physiology
    Title Assessment of Different Methods of Inhalation from Salbutamol Metered Dose Inhalers by Urinary Drug Excretion and Methacholine Challenge.
    Date April 2006
    Journal British Journal of Clinical Pharmacology
    Excerpt

    Methods to determine the lung delivery of inhaled bronchodilators from metered dose inhalers include urinary drug excretion 30 min post inhalation and methacholine challenge (PD20). We have compared these two methods to differentiate lung delivery of salbutamol from metered dose inhalers using different inhalation methods.

    Title Pregnancy in Kyphoscoliosis: Benefit of Non-invasive Ventilatory Support.
    Date October 2005
    Journal Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology
    Title A 29-year-old Male with Malaise, Chest Pain and Night Sweats.
    Date August 2005
    Journal The European Respiratory Journal : Official Journal of the European Society for Clinical Respiratory Physiology
    Title Unexpected Nocturnal Hypoxia in Patients with Acute Stroke.
    Date November 2003
    Journal Stroke; a Journal of Cerebral Circulation
    Excerpt

    Patients who have had a stroke are at risk of hypoxia through alterations in the central regulation of respiration, through aspiration, and through respiratory muscle weakness. Sleep-related breathing disorders are common and may lead to episodes of nocturnal hypoxia even when daytime oxygenation is normal. The aim of this study was to assess the prevalence of unexpected nocturnal hypoxia in stroke patients.

    Title Pulmonary Toxicity of Cardiac Drugs.
    Date October 2003
    Journal Drugs of Today (barcelona, Spain : 1998)
    Excerpt

    Pulmonary damage caused by the undesired effects of cardiac drugs is being increasingly recognized. With the symptoms of breathlessness, wheeze, cough and hemoptysis common to both cardiac and respiratory diseases, an adverse pulmonary reaction to a cardiac drug may be mistakenly attributed to deterioration of cardiac status. This is especially true if the onset of symptoms are subacute or chronic. The pulmonary side effects of cardiac drugs are varied and may actually mimic the disease being treated. It is essential that the clinician remain highly suspicious that new and unexplained pulmonary symptoms and signs may be drug-related. Most of the side effects are reversible with early drug cessation and subsequent challenge should be avoided if possible.

    Title Factors Influencing the Distribution of Myxobolus Cerebralis, the Causative Agent of Whirling Disease, in the Cache La Poudre River, Colorado.
    Date October 2002
    Journal Diseases of Aquatic Organisms
    Excerpt

    Oligochaetes, triactinomyxons (TAMs), and age-0 trout were sampled in the upper Cache la Poudre River, Colorado, to determine the distribution of Myxobolus cerebralis during 1997 and 1998. Densities of the intermediate host, the oligochaete Tubifex tubifex, were 3.5 orders of magnitude higher in the M. cerebralis-infected Poudre Rearing Unit (PRU) trout rearing ponds than at any of the river sampling reaches. Oligochaetes, including T. tubifex, were rare in the river (1 oligochaete m(-2)), except in a few stream side alcoves and eddies (50 oligochaete m(-2)). Species composition of oligochaetes in the river reaches was more diverse than in the PRU. Tubifex tubifex constituted 50% or less of the oligochaete community in the river and 98% in the PRU. Infection rates of T tubifex were 1% in the area above the PRU, 2% in the PRU, and 6% below the PRU. An increased M. cerebralis intensity of infection in age-0 trout below the PRU could not be attributed entirely to the high numbers of TAMs in its effluent (3.7 TAMs l(-1)). Low densities of TAMs ranging from 0 to 0.2 TAMs l(-1) were found in the river reaches, yet nearly all of the age-0 trout were infected soon after emergence. This suggests that very few TAMs, as measured by filtration, need be present in the water column to bring about infection in the majority of trout present. This also indicates that the parasite can persist and potentially cause reduced juvenile trout recruitment in cold, oligotrophic, sediment poor, high-gradient streams.

    Title Using Spirometry in General Practice.
    Date August 2001
    Journal The Practitioner
    Title A Breathless Female.
    Date August 2000
    Journal The European Respiratory Journal : Official Journal of the European Society for Clinical Respiratory Physiology
    Title Reproducibility of Bronchodilator Response for a Reservoir Dry Powder Inhaler Following Routine Clinical Use.
    Date March 2000
    Journal The Journal of Asthma : Official Journal of the Association for the Care of Asthma
    Excerpt

    The performance of dry powder inhaler (DPI) devices, particularly reservoir DPIs, may be influenced by environmental conditions. This study compared the bronchodilator efficacy and in vitro aerosol characteristics of salbutamol, delivered via a novel reservoir DPI (Clickhaler) and a conventional pressurized metered-dose inhaler (MDI) before and after use of the DPI in clinical practice. Following a screening visit, patients received cumulative doses of salbutamol (100, 200, and 400 microg) via DPI or MDI on separate days in a double-blind, crossover design before and after a 4-week period, during which the DPI was used as the patients' first-line bronchodilator. Lung function responses (forced expiratory volume in 1 sec [FEV1], forced vital capacity [FVC], and peak expiratory flow [PEF]) to salbutamol delivered by DPI and MDI and in vitro aerosol characteristics were not significantly different before and after the period of DPI patient use. DPI performance, assessed in vivo and in vitro, is maintained following an extended period of patient use.

    Title General Practice Spirometry in North Staffordshire.
    Date July 1999
    Journal Monaldi Archives for Chest Disease = Archivio Monaldi Per Le Malattie Del Torace / Fondazione Clinica Del Lavoro, Irccs [and] Istituto Di Clinica Tisiologica E Malattie Apparato Respiratorio, UniversitĂ  Di Napoli, Secondo Ateneo
    Excerpt

    Spirometry is suggested, in North American and European guidelines, to be the most important measurement of lung function for the management of patients with chronic obstructive pulmonary disease (COPD). The aim of this study was to determine attitudes to and utilization of spirometry in general practices within North Staffordshire. All 95 practices in North Staffordshire were surveyed by telephone, using a standard proforma. Eighty-four practices (48 fundholding) containing 224 general practitioners (GPs) and serving 472,420 patients, agreed to take part. The survey was completed by practice nurses on 63 occasions, GPs on 14 and practice managers on seven. Eighteen practices possessed a spirometer, although eight did not use it. The measurements were performed by doctors in five of the practices, nurses in two and by both in three. Only two users had received formal training in the performance and interpretation of spirometry. Of the three practices using pneumotachograph spirometers, none knew how often the machine should be calibrated. Spirometry was used by five practices for diagnosis (although only four used it to determine forced expiratory volume in one second (FEV1)), three for monitoring and only one for bronchodilator reversibility testing. Although 44 (52%) practices thought that, ideally, spirometry should be available in the practice, only 10 of the 18 who had spirometers were currently providing this facility. Furthermore, the poor training puts into question the validity of some of the results obtained. Open access to hospital spirometry is one method of addressing these problems, and 73 (87%) practices, including 16 who already owned a spirometer, said that they would use such a service.

    Title Tuberculin Skin Testing.
    Date July 1999
    Journal Chest
    Title Medical Students' Knowledge of Smoking.
    Date June 1999
    Journal Thorax
    Title Most Practices Would Use Open Access Spirometry in Hospitals.
    Date August 1998
    Journal Bmj (clinical Research Ed.)
    Title Sedation in Fiberoptic Bronchoscopy.
    Date May 1997
    Journal Chest
    Title Non-invasive Ventilation for Acute Exacerbations of Chronic Obstructive Pulmonary Disease.
    Date March 1997
    Journal Bmj (clinical Research Ed.)
    Title Compliance with Oral Corticosteroids During Steroid Trials in Chronic Airways Obstruction.
    Date September 1996
    Journal Thorax
    Excerpt

    Corticosteroid trials are an important part of the assessment of patients with chronic airways obstruction, but false negative results will occur if the treatment is not taken. To determine compliance low dose phenobarbitone has been used as a marker.

    Title Pulmonary Tuberculosis and Steroids.
    Date June 1996
    Journal Chest
    Title Low Incidence of Red Cell and Hla Antibody Formation by Bone Marrow Transplant Patients.
    Date May 1996
    Journal Transfusion
    Excerpt

    BACKGROUND: Bone marrow transplant (BMT) patients, although immunosuppressed, are at risk for the development of red cell (RBC) and HLA antibodies, and they often are given filtered blood in an effort to prevent the latter complication. This study attempts to determine the rate of formation and the specificity of both RBC and HLA alloantibodies in this patient population. STUDY DESIGN AND METHODS: BMT patients (148 received autologous marrow; 45 received allogeneic marrow) from an 18-month period, including patients with leukemia (57 patients), lymphoma (54), breast cancer (68), myeloma (8), myelodysplastic syndrome (5), and aplastic anemia (1), were studied to determine the rate of alloantibody formation to RBC and HLA antigens. A total of 2,410 RBC antibody screens were performed. The patients received 3,921 packed RBCs and 5,915 single-donor platelet units; all were irradiated and administered via white cell-reduction filters. RESULTS: Seven (3.6%) of 193 patients had RBC antibodies upon hospital admission. Four (2.1%) of 193 developed RBC antibodies during the course of BMT: 3 patients had one RBC antibody and 1 patient had two RBC antibodies. RBC antibodies included anti-E (n = 2), anti-M (n = 1), anti-Jkb (n = 1), and anti-Lu14 (n = 1). Thus, 98 percent of patients (189/193) did not develop new (182/186) or additional (7/7) RBC antibodies during BMT. BMT patients were also screened weekly for HLA antibody formation (60-cell panel). Upon admission, 170 (85%) patients were negative. Of these, 8 (4.7%) developed persistent HLA antibodies (mean panel-reactive antibody score, 33 +/- 29%) and 9 (5.3%) were variably positive. Thus, in our setting and population, RBC antibody formation was 0.1 percent per unit transfused, and the HLA alloimmunization rate was 5 to 10 percent. CONCLUSION: As RBC antibody screens are done every Monday, Wednesday, and Friday on this BMT service and as RBC antibody formation is low in these patients, screening for unexpected antibodies might be possible on a more infrequent basis. Also, the rate of HLA alloimmunization in this population receiving filtered blood components is low.

    Title Childhood Cancer in Britain: the National Registry of Childhood Tumours and Incidence Rates 1978-1987.
    Date February 1996
    Journal European Journal of Cancer (oxford, England : 1990)
    Excerpt

    The National Registry of Childhood Tumours contains population-based data on childhood cancers diagnosed throughout Great Britain from 1962 onwards. This paper describes the methodology of the Registry, presents incidence rates for 1978-1987 and describes other uses of the data. Total age-standardised annual incidence was 118.3 per million. The most frequent diagnostic groups were leukaemias (age-standardised rate 39.8), brain and spinal tumours (27.0), lymphomas (11.1), sympathetic nervous system tumours (8.3), kidney tumours (7.7) and soft-tissue sarcomas (7.5). Incidence rates were similar to those reported from other Western industrialised countries. The data are also used for a wide range of epidemiological and other studies. These include analyses of geographical variations in incidence, trends in survival, health of long-term survivors and their offspring and the genetics of childhood cancer. Information is frequently provided for clinicians and research workers, and series of specific types of cancer are compiled for further study. The Registry depends for the completeness and accuracy of its data on a wide range of organisations and individuals, and it is essential that this cooperation continues if the Registry is to be maintained.

    Title Nocturnal Hypoxaemia in Cystic Fibrosis.
    Date November 1995
    Journal Respiratory Medicine
    Title Sedation in Fibreoptic Bronchoscopy.
    Date July 1995
    Journal Bmj (clinical Research Ed.)
    Title Rectal Metastases from Small Cell Lung Cancer.
    Date June 1995
    Journal Respiratory Medicine
    Title Analgesia and Sedation in Fiber Optic Bronchoscopy.
    Date May 1995
    Journal Respiratory Medicine
    Title Does Sedation Help in Fibreoptic Bronchoscopy?
    Date January 1995
    Journal Bmj (clinical Research Ed.)
    Title Pleural and Pericardial Fibrosis After Ergotamine Therapy.
    Date August 1994
    Journal Respiratory Medicine
    Title Nasal Surgery for Snoring.
    Date June 1994
    Journal Clinical Otolaryngology and Allied Sciences
    Excerpt

    Snoring is a common disorder usually presenting to otolaryngologists. Most patients who have any nasal symptoms would normally be offered nasal surgery, but there is a significant incidence of failure to control the snoring (approximately 25%). We analysed a group of patients, all having nasal surgery for snoring, with the aim of assessing whether any pre-operative factors would predict success or failure of the surgery. We found symptoms of excessively loud snoring, witnessed apnoeic episodes and hypersomnolence, or obstructive sleep apnoea diagnosed by overnight oxygen saturation measurements to be statistically associated with failure of nasal surgery to improve snoring.

    Title Changes in Nocturnal Oximetry After Treatment of Exacerbations in Cystic Fibrosis.
    Date November 1993
    Journal Archives of Disease in Childhood
    Excerpt

    Sleep related arterial oxygen desaturation has been described in clinically stable young adults with cystic fibrosis. The incidence and severity of nocturnal oxygen desaturation in children during infective exacerbations and the changes that occur with treatment were examined. Forty five children with proved cystic fibrosis, median age 8.9 years, admitted to the Regional Cystic Fibrosis Unit underwent clinical evaluation, spirometry, and measurement of peak flow and nocturnal oxygen saturation on admission and after 10 days' treatment. There was a significant improvement in all the above measurements, with the averaged overnight saturation changing from a mean (SD) 92.7 (2.7)% to 94.3 (2.0)%, mean (SE) difference 1.58 (0.37). The time spent with a saturation 4% or more below their clinic value showed a marked improvement from 122 (152) minutes on the first night to 21 (30.7) on the second, mean (SE) difference 101 (22.4). Eight young children could not perform pulmonary function tests, all desaturated on the admission night. Nocturnal hypoxaemia is a common finding in young cystic fibrosis patients during infective exacerbations but improves with treatment. Overnight oximetry is simple to perform, well tolerated, and identifies patients with marked nocturnal desaturation.

    Title Pulmonary and Cutaneous Vasculitis Following Hepatitis B Vaccination.
    Date August 1993
    Journal Thorax
    Title Salbutamol Output from Two Jet Nebulizers.
    Date June 1993
    Journal Respiratory Medicine
    Excerpt

    Nebulized bronchodilators are commonly used in the management of patients with airflow obstruction, although there is little information available on the bronchodilator output from the nebulizer unit. We have examined the fluid and salbutamol outputs of a single jet nebulizer from two commercial manufacturers at 1 min intervals up to 12 min. The drug and fluid output continued throughout the study period, with a greater fluid output leading to an increase in the concentration of salbutamol remaining within the nebulizer unit. This suggests that weight change is not a good indicator of drug output. Furthermore, there was a marked difference in peak salbutamol output between the two nebulizer units, being 2.9 mg (55%) with the Micro-Neb unit and 1.98 mg (38.7%) with the System 22 unit.

    Title Pancoast Syndrome: an Unusual Presentation of Adenoid Cystic Carcinoma.
    Date April 1993
    Journal The European Respiratory Journal : Official Journal of the European Society for Clinical Respiratory Physiology
    Excerpt

    We report on a patient with primary pulmonary adenoid cystic carcinoma presenting with Pancoast syndrome. Pancoast syndrome has not previously been described with this tumour. Other unusual features of this case include the peripheral origin and mediastinal involvement, with lack of proximal endobronchial spread.

    Title Time of Development of Tuberculosis in Contacts.
    Date January 1993
    Journal Respiratory Medicine
    Title The Glory of Woman or Love, Marriage and Maternity. 1896.
    Date December 1992
    Journal Journal of Human Lactation : Official Journal of International Lactation Consultant Association
    Title Asthma and Wheezing.
    Date August 1992
    Journal Lancet
    Title Accuracy and Significance of Scoring Hypopneas.
    Date July 1992
    Journal Sleep
    Excerpt

    We previously reported that the best definition of hypopneas in the sleep apnea/hypopnea syndrome (SAHS) is based on reduction in thoracoabdominal movement. However, the repeatability of scoring hypopneas from thoracoabdominal movement has not been assessed, nor has the need to record flow as well as thoracoabdominal movement. Thus, two polysomnographers independently scored both apneas and hypopneas on all-night polysomnograms of patients with SAHS. There was close agreement between the polysomnographers for the number of hypopneas (r = 0.98; mean difference 11%) and for the number of apneas (r = 0.99; mean difference 8%). The agreement was similar for the durations of both hypopneas (r = 0.99; mean difference 13%) and apneas (r = 0.99; mean difference 11%). There was also close agreement between the total number of respiratory events scored with and without reference to the flow signal (r = 0.99; mean difference 1.4%) with a maximum under-recognition of 18 events per night in a subject with 237 apneas per night. Thus, hypopneas can be scored reproducibly. In addition, the value of always recording and scoring flow as well as thoracoabdominal signals is questioned.

    Title 'smoke-bomb' Pneumonitis.
    Date July 1992
    Journal Respiratory Medicine
    Title Corticosteroids and Tuberculosis.
    Date December 1991
    Journal Bmj (clinical Research Ed.)
    Title Obstructive Sleep Apnoea in Adults Presenting with Snoring.
    Date December 1991
    Journal Clinical Otolaryngology and Allied Sciences
    Excerpt

    Snoring is a common disorder, and may be associated with obstructive sleep apnoea, although there is little published information on the incidence of apnoea in snorers. This study aimed to assess the upper airway and to relate the findings to sleep study data in a population of patients referred by their general practitioners with loud snoring. Each patient had a full history, weight and height measurements, nasal examination, rhinomanometry, peroral grading of the oropharyngeal features, and fibreoptic pharyngoscopy with a modified Muller manoeuvre, followed by a sleep study. The results in our group of 35 patients demonstrate a high incidence of obstructive sleep apnoea (46%). Factors which correlated well with apnoea were excessively loud snoring, a narrow oropharynx, and marked obesity; 94% of patients with one or more of these features had evidence of sleep apnoea.

    Title Salmeterol Rash.
    Date June 1991
    Journal Lancet
    Title Vasculitis Related to Hepatitis B Vaccine.
    Date February 1991
    Journal Bmj (clinical Research Ed.)
    Title How Well Do the General Public Read Heaf Skin Tests?
    Date November 1990
    Journal Respiratory Medicine
    Excerpt

    The ability of 550 consecutive patients attending a tuberculosis clinic to grade their own Heaf skin test reaction, using an illustrated card designed for such self-reporting, was assessed. Individuals were asked to grade their own Heaf skin reaction and at the same attendance the reaction was formally graded by a respiratory health visitor. There was overall excellent agreement with only 58 (10.5%) disparate gradings. We conclude that self-reporting of Heaf skin tests using an illustrated card is simple, usually accurate and may preclude an otherwise unnecessary early reattendance. This study also validates an often used system of self-reporting.

    Title The Use of Probenecid to Increase the Serum Amoxycillin Levels in Patients with Bronchiectasis.
    Date August 1990
    Journal Respiratory Medicine
    Excerpt

    Amoxycillin 3 g twice daily is effective in treating patients with bronchiectasis who have daily purulent sputum. However, it is a relatively expensive treatment if used for prolonged periods. This pilot study in six patients with bronchiectasis showed that the concurrent administration of probenecid with a smaller dose of amoxycillin produced similar clinical responses and pharmacokinetic profiles to those found with high dose amoxycillin alone. The amount of amoxycillin used was reduced by two thirds, producing a potential saving of approximately pound 1000 per patient per annum in those taking long term treatment.

    Title Chemosensitivity Testing of Human Gliomas Using a Fluorescent Microcarrier Technique.
    Date July 1990
    Journal Journal of Neuro-oncology
    Excerpt

    This report describes a new fluorescent microcarrier cytostasis assay. Human glioma cell lines and primary cultures were attached to microcarrier tissue culture beads and treated with various chemotherapeutic drugs. After treatment, the cells were labelled with two vital fluorescent dyes in order to measure cellular viability. The uptake of hydroethidine and Hoechst 33342 was evaluated alone and in combination as probes for determining metabolic activity and cellular proliferation. Hydroethidine was found to be superior when compared to trypan blue and tritiated thymidine. The use of the microcarrier technique allows for the direct cellular measurement of fluorescence without the need of extensive extraction procedures. The fluorescent assay is a sensitive, rapid and an effective way to screen for potential antiproliferative compounds.

    Title Clinical Features of the Sleep Apnoea/hypopnoea Syndrome.
    Date February 1990
    Journal The Quarterly Journal of Medicine
    Excerpt

    The sleep apnoea/hypopnoea syndrome has been reported to be rare in Britain. We have offered a clinical service for the diagnosis and treatment of this condition in Scotland for four years during which there has been an increase in referral rate from 19 patients in year 1 to 61 in year 4. We report the clinical features of the 80 patients. Seventy-eight snored and 70 were somnolent, each falling asleep at least once each day when not in bed. Ten reported falling asleep whilst driving or flying. Twenty-nine slept restlessly, 28 were not refreshed by sleep and 21 had nocturnal choking attacks. Ankle swelling was reported by 26 and 25 were hypertensive (diastolic BP greater than 95 mmHg). Two had hyperthyroidism and one acromegaly. Nineteen have been successfully treated by continuous positive airway pressure therapy at home. The data suggest that regional centres should be established in Britain to diagnose and treat this condition.

    Title Optimal Duration of Radiological Follow-up for Tuberculosis Contacts.
    Date February 1990
    Journal Respiratory Medicine
    Excerpt

    Eight hundred and sixty Heaf test positive contacts of cases of tuberculosis in Lothian were offered screening with chest radiographs for the recommended period of two years. In spite of the use of standard methods of encouragement to attend, only 309 (35.9%) completed the two year period of follow-up. Seven cases of tuberculosis (representing a crude rate of eight cases per 1000 contacts) were detected radiologically, all within 3 months of entering the screening programme. In view of the poor compliance with the extended period of follow-up and the detection of all cases early in the screening process, we recommend that, at least in Lothian, radiological follow-up of Heaf positive tuberculosis contacts need only be applied for a period of 6 months.

    Title Transient Impairment of Renal Function After Streptokinase Therapy.
    Date January 1990
    Journal Lancet
    Title Hypercalcemia in Atypical Bronchial Carcinoid Tumors.
    Date November 1989
    Journal Chest
    Excerpt

    Atypical carcinoid tumors of the bronchial tree are uncommon. Their tendency to metastasize is well recognized, characteristically producing osteoblastic bone deposits without disturbance of calcium homeostasis. We report two patients who presented with hypercalcemia and osteolytic bone metastases following surgical removal of atypical bronchial carcinoid tumors. In one of the patients, chemotherapy induced remission and controlled the hypercalcemia.

    Title Steroid Aerosols and Cataract Formation.
    Date November 1989
    Journal Bmj (clinical Research Ed.)
    Title Respiratory Symptoms and Pulmonary Impairment in Male and Female Subjects with Pottery Workers' Silicosis.
    Date November 1989
    Journal The Annals of Occupational Hygiene
    Excerpt

    Respiratory symptoms, smoking habit, lung function and radiological category of silicosis were assessed in 276 present and former pottery workers who were receiving industrial disablement benefit for silicosis. There were 140 females and 136 males. The proportion with conglomerate disease (massive fibrosis) was similar in both sexes. The FEV1 declined with increasing X-ray category of silicosis irrespective of smoking habit and was most marked in subjects with symptomatic chronic bronchitis. In females who had never smoked the average decline of FEV1 in those with simple silicosis was 18 ml year-1 and for those with conglomerate disease 38 ml year-1. Symptomatic chronic bronchitis was common and only partly related to smoking, occurring in 69% of 101 nonsmoking female silicotic patients. No significant changes were observed in vital capacity, lung volume or transfer factor for carbon monoxide.

    Title Sleep Apnoea.
    Date October 1989
    Journal British Journal of Diseases of the Chest
    Title Nebuhaler or Nebulizer for High Dose Bronchodilator Therapy in Chronic Bronchitis: a Comparison.
    Date October 1989
    Journal British Journal of Diseases of the Chest
    Excerpt

    We have compared the clinical efficacy of high dose terbutaline sulphate (10 mg four times daily) delivered by either a Nebuhaler or jet nebulizer in 13 patients with chronic bronchitis in a 2-week, open, crossover study. Both treatment regimens improved run-in symptom scores but no significant changes were recorded in peak flow and spirometry. Side-effects were more common with the Nebuhaler and more patients preferred the nebulizer. However, the Nebuhaler is an alternative therapeutic option for delivery of high doses of bronchodilators in patients with chronic bronchitis.

    Title Peripheral Nerve Function in Patients with Chronic Bronchitis Receiving Almitrine or Placebo.
    Date September 1989
    Journal Thorax
    Excerpt

    A double blind prospective study of the effect of almitrine bismesylate and placebo on peripheral-nerve function was carried out in 12 patients with chronic bronchitis and arterial hypoxaemia (mean (SD) FEV1% predicted 38 (16), arterial oxygen tension (PaO2) 7.56 (0.76) kPa). Of the seven patients who took placebo, none developed symptoms or signs of peripheral neuropathy. One patient who had abnormal lower limb sensory nerve conduction initially showed improvement of sensory conduction but deterioration in motor conduction during the 12 month study period. Two further patients developed some slowing of motor conduction velocities in their right lateral popliteal nerve. Five patients received almitrine and all showed an improvement in PaO2 (mean from 7.0 to 7.9 kPa). None had symptoms or signs of peripheral neuropathy on entry to the study; one patient had evidence of impaired nerve conduction on electrophysiological testing. Three patients developed symptoms and signs of peripheral neuropathy during the 12 months of the study and a fourth developed peripheral neuropathy at 18 months, having continued to receive almitrine. Studies of nerve physiology showed abnormalities in the lower limbs of all four patients. Recovery was poor, possibly because of the long half life of almitrine. The studies suggest that almitrine may precipitate peripheral neuropathy in patients with chronic obstructive pulmonary disease. Patients should be warned of this potential complication so that the drug can be stopped as soon as symptoms develop.

    Title Immunocytochemical Study of Transforming Growth Factor Expression in Benign and Malignant Gliomas.
    Date May 1989
    Journal The American Journal of Pathology
    Excerpt

    Immunocytochemical studies using polyclonal antibodies to epidermal growth factor (EGF) and transforming growth factor (TGF) alpha and beta were performed on 20 cases of human gliomas. EGF immunoreactive material was detected in both benign and malignant glial tumors. In addition, EGF immunoreactive material was detected in normal brain. TGF-beta was detected in both benign and malignant tumors, but was not detected in normal brain. In contrast, TGF-alpha was highly conserved in its expression, occurring predominantly in malignant compared with benign or normal brain tissue (P less than 0.0001). In malignant gliomas, glioblastomas contained 76% TGF-alpha reactivity (immunoreactive product), and anaplastic types contained 85% reactivity. Benign gliomas contained only 13% TGF-alpha reactivity. These findings support the role of TGF-alpha as an oncoprotein marker in brain neoplasms.

    Title Ganglioglioma, a Malignant Tumor? Correlation with Flow Deoxyribonucleic Acid Cytometric Analysis.
    Date March 1989
    Journal Neurosurgery
    Excerpt

    This study describes the flow cytometric deoxyribonucleic acid (DNA) analysis of a resected ganglioglioma. The initial histopathological analysis revealed a benign tumor characterized by a predominance of mature ganglion cells. The flow cytometric DNA analysis of the necrotic areas, however, demonstrated an aneuploid population of cells. Further examination by histological analysis of the tumor revealed both benign and atypical foci. The retrospective DNA analysis performed from paraffin sections of tissue with benign-histological findings demonstrated euploid populations of cells consistent with a benign, slow-growing lesion. In contrast, DNA analysis performed from tissue with atypical histological findings revealed aneuploid populations of cells consistent with a malignant phenotype. Our analysis provides additional data supporting the existence of tumor progression in some gangliogliomas. Results support the concept of tumor cell heterogeneity and the importance of adequate tumor sampling. The finding of aneuploid populations with unfavorable histology further supports the use of flow cytometry as an adjunct method in assessing tumor biology.

    Title Bacteraemic Haemophilus Influenzae Pneumonia.
    Date March 1989
    Journal The European Respiratory Journal : Official Journal of the European Society for Clinical Respiratory Physiology
    Excerpt

    Bacteraemia with Haemophilus pneumonia is uncommon. To determine its incidence and features case notes of patients in whom Haemophilus spp. were isolated from blood and pleural fluid over a five-year period were reviewed. Eight adult patients with H. influenzae bacteraemia were identified, five of whom had pneumonia on clinical and radiographic criteria. Only one patient had a predisposing factor, chronic obstructive lung disease. Two patients had beta-lactamase producing isolates, one of whom developed an empyema, following treatment with ampicillin, which required surgical drainage. Four patients were elderly, aged 69-80 yrs and were clinically in shock at the time of diagnosis. Seven of the eight patients survived.

    Title Legionella Pneumonia Complicating Wegener's Granulomatosis.
    Date December 1988
    Journal Chest
    Excerpt

    We describe a previously healthy man who presented with features consistent with Wegener's granulomatosis. While undergoing investigation, he developed acute respiratory failure, thought to represent progression of his vasculitis. Open lung and sinus biopsies were performed to obtain the diagnosis. Vasculitis was confirmed on the paranasasl biopsy, and the lung biopsy showed pneumonia due to Legionella pneumophila, an association not previously reported in Wegener's granulomatosis. If immunosuppressive therapy had been started without making the diagnosis of Legionella pneumonia on lung biopsy, the patient might well have succumbed to the infection.

    Title Almitrine and Peripheral Neuropathy.
    Date October 1988
    Journal Lancet
    Title Intrathoracic Infections Due to Eikenella Corrodens.
    Date September 1988
    Journal Thorax
    Title Mr Evaluation of Hydrocephalus.
    Date October 1987
    Journal Ajr. American Journal of Roentgenology
    Excerpt

    An analysis of sagittal T1-weighted MR studies was performed in 23 patients with hydrocephalus, 58 patients with atrophy, and 100 normal patients. The average mamillopontine distance was 1.15 cm for the normal group, 1.2 cm for patients with atrophy, and 7.5 mm for patients with hydrocephalus. A reduction of the mamillopontine distance below 1.0 cm was found in 22 patients with hydrocephalus, 5 patients with atrophy, and 15 normal patients. Dilatation of the anterior third ventricle was noted in 21 patients in the hydrocephalus group and in none of the patients in the atrophy and normal groups. The average thickness of the corpus callosum at the level of the foramen of Monro was 6 mm in normal subjects and was reduced below 6 mm in 16 of the hydrocephalus patients. Smooth elevation of the corpus callosum was noted in 20 hydrocephalus patients, in 2 patients with atrophy, and in none of the normal patients. MR improves the accuracy of diagnosis in patients with hydrocephalus both because of its ability to show small obstructing lesions that are not depicted by CT and because the mass effect of the distended supratentorial ventricles produces anatomic changes that are delineated with precision by MR.

    Title Skin Necrosis Following Haemaccel.
    Date March 1987
    Journal Postgraduate Medical Journal
    Excerpt

    Haemaccel is a plasma substitute in frequent clinical use with a low incidence of side effects. I report a patient who developed necrotic blisters following two Haemaccel-insulin infusions, a previously undescribed complication. The rationale behind carrier solutions for insulin therapy is discussed and some of the problems associated with Haemaccel use are considered. I conclude by urging caution in the use of Haemaccel as a carrier solution for insulin therapy.

    Title Alpha 1 Antitrypsin Deficiency Due to Mmaltonz Phenotype: Case Report and Family Study.
    Date January 1987
    Journal Thorax
    Title Smoking, Drinking and Polycythemia.
    Date November 1986
    Journal British Medical Journal (clinical Research Ed.)
    Title Intradural Dermoid Cyst. A Case Report.
    Date March 1985
    Journal Spine
    Excerpt

    Intraspinal tumors must be included in the long list of differential diagnosis in the patient presenting with the low-back radicular pain syndrome. These tumors are classified easily by their relationship to the dura, and the type of compression readily discerned by myelography. Intradural dermoid cysts account for 1% of intraspinal tumor, but prompt, meticulous, surgical intervention may result in an excellent outcome.

    Title Acute Anterior Spinal Epidural Abscess.
    Date October 1984
    Journal Southern Medical Journal
    Excerpt

    We have reported a case of acute spinal epidural abscess, an uncommon cause of pain in the neck and back. The typical progression from spinal ache to paralysis may be halted if the diagnosis is suspected and prompt radiologic and surgical interventions are initiated.

    Title Effect of Pressure on Cerebrospinal Fluid Absorption in Cats, Baboons, and Humans: Comparison of the Linear and Logarithmic Models.
    Date June 1982
    Journal Neurosurgery
    Excerpt

    To circumvent time constraints inherent in indicator clearance measurements of cerebrospinal fluid absorption, investigators have used the relationship between CSF pressure at steady state and the rate of infusion of mock CSF, in both patients and experimental animals, to evaluate the bulk absorption rate of CSF. This latter approach required mathematical model of the effect of CSF pressure on CSF bulk flow. Two such models--a fixed resistance and a variable resistance model of CSF flow through arachnoid villi--have been used in both clinical and laboratory settings. In this study, the relationship between steady state CSF pressure and mock CSF infusion rate was assessed using both mathematical models. We studied two patients, three cats, and seven baboons. Values of CSF outflow resistance calculated according to both models as well as other parameters of CSF bulk flow estimated on the basis of both models were all in the range expected from other studies. The data from these experiments do not provide justification for preferring one model over the other. Depending on the experimental or clinical situation, some of the assumptions behind both models may not be valid. Multiple direct measurements of the rates of CSF absorption and formation over a wide range of CSF pressures in individual subjects will be necessary to validate either the variable or the fixed resistance model or to suggest a more appropriate model. Until such information is available, it is probably reasonable to use both approaches for the analysis of mock CSF infusion data. CSF bulk flow parameters calculated on the basis of either the variable or the fixed resistance model should never be taken as absolute, but should be evaluated critically in the context of the clinical or experimental situation.

    Title Effect of Neonatal Androgenization on the Lhrh Response of Dispersed Pituitary Cell Cultures.
    Date May 1982
    Journal Archives of Andrology
    Excerpt

    To determine if neonatal androgenization affects pituitary luteinizing hormone releasing hormone (LHRH) response, female rats were androgenized 24-48 hr following birth. Intact male and female litter mates were maintained for comparison. At 83 days of age, randomly selected males and androgenized females were gonadectomized. At 90 days of age, pituitary cell cultures were begun. On day 3 the cells were challenged with LHRH. Gonadotropin response was determined by radioimmunoassay (RIA). Basal gonadotropin release from androgenized female cells was similar to male cells, while the pattern and magnitude of the LHRH response was similar to intact female cells. After castration, the pattern and magnitude of the LHRH response of the androgenized female pituitary was most like the male. Androgenized female cells exhibited mixed male and female LHRH response.

    Title Transsphenoidal Surgery on the Pituitary.
    Date September 1981
    Journal The American Surgeon
    Excerpt

    Transseptal transsphenoidal hypophysectomy has evolved into a low mortality/morbidity procedure in properly selected patients. Preoperative evaluation is becoming increasingly accurate with the rapid advances in computerized axial tomography. With the lowering of operative morbidity, indications for the transsphenoidal approaches to the sella are broadening. Caution must be used when approaching pituitary tumors with extensive suprasellar components by the transsphenoidal route.

    Title Adult Arteriovenous Malformation of the Vein of Galen: a Case Report with Pre- and Postoperative Computed Tomographic Findings.
    Date February 1981
    Journal Neurosurgery
    Title Structural and Developmental Aspects of Hepatic Glucokinase.
    Date December 1980
    Journal Archivos De BiologĂ­a Y Medicina Experimentales
    Title Apparent 'glucokinase' Activity in Non-hepatic Tissues Due to N-acetyl-d-glucosamine Kinase.
    Date November 1980
    Journal Biochimica Et Biophysica Acta
    Excerpt

    1. Electrophoretic examination of tissue extracts from rat intestinal mucosa, kidney, lung, spleen, mammary gland, adipose tissue, heart muscle and placenta in agarose gels did not reveal the presence of any glucokinase (ATP:D-glucose 6-phosphotransferase, EC 2.7.1.2) activity corresponding to that present in rat liver. 2. All these tissues do contain an enzyme that possesses very high-Km glucose-phosphorylating activity but which has a slightly lower electrophoretic mobility than glucokinase and can be separated from it by various means. 3. This phosphotransferase activity is due to N-acetyl-D-glucosamine kinase (ATP:2-acetamido-2-deoxy-D-glucose 6-phosphotransferase, EC 2.7.1.59), which has been partialyy purified from intestinal mucosa tissue and shown to have similar kinetic properties to the same enzyme previously purified more extensively from liver and kidney. 4. It is suggested that many of the effects reported in the literature of 'glucokinase' activity in non-hepatic tissues are probably due to N-acetyl-D-glucosamine kinase.

    Title Factors That Prevent the Premature Appearance of Glucokinase in Neonatal Rat Liver.
    Date September 1980
    Journal The Biochemical Journal
    Excerpt

    1. The physiological factors that prevent the precocious appearance of glucokinase activity in the 13-day-old rat that can be induced by oral glucose administration were explored. 2. Evidence is presented that the galactose component of milk sugar is inhibitory. In the absence of this inhibitory galactose, the amount of glucose necessary to effect appreciable induction is greater than that present in milk. 3. The induction is prevented both by administration of mannoheptulose, which inhibits insulin release, and by excess insulin; the amount of insulin available therefore seems to be critical. 4. The inhibition of induction by galactose does not appear to be via competition with glucose but by enhancing insulin release and thereby making this excessive. The relative amounts of glucose and insulin appear to be important in regulating glucokinase induction. 5. The precocious induction of glucokinase by glucose is inhibited by simultaneous treatment with approriate amounts of adrenaline, glucagon, dibutyryl cyclic AMP or isoprenaline but not by vasopressin or angiotensin II. 6. No single cause of glucokinase induction in neonatal rat liver can be recognized. The process is subject to regulation by many factors at a time subsequent to when competence to synthesize the enzyme has been established.

    Title The Isolation and Preliminary Characterization of N-acetyl-d-glucosamine Kinase from Rat Kidney and Liver.
    Date August 1980
    Journal The Biochemical Journal
    Excerpt

    1. Procedures for the extensive purification in high yield of N-acetyl-D-glucosamine kinase from rat liver and kidney are described. The separation of this enzyme from hepatic glucokinase depended primarily on their differing behaviour on an affinity column of Sepharose--N-(6-aminohexanoyl)-2-amino-2-deoxy-D-glucopyranose. 2. This N-acetyl-D-glucosamine kinase also catalyses the phosphorylation of N-acetyl-D-mannosamine and, at a lower rate, several other sugar analogues, including D-glucose. 3. A comparison of the behaviour of the enzyme during gel filtration and electrophoresis in sodium dodecyl sulphate/polyacrylamide gels suggests that N-acetyl-D-glucosamine kinase is a symmetrical dimer of mol.wt. 80000.

    Title Kinetic Characterization of N-acetyl-d-glucosamine Kinase from Rat Liver and Kidney.
    Date August 1980
    Journal The Biochemical Journal
    Excerpt

    1. Under normal assay conditions the N-acetyl-D-glucosamine kinases from rat liver and kidney show a pH-dependent lag phase before reaching a steady state, which is probably due to reversible dissociation of the dimeric enzyme. 2. The enzyme catalyses the phosphorylation of N-acetyl-D-glucosamine, N-acetyl-D-mannosamine and D-glucose at pH 7.5, with apparent Km values of 0.06, 0.95 and 600 mM respectively for the enzyme from liver and 0.04, 1.0 and 410 mM respectively for the kidney enzyme. It is strongly inhibited by ADP. 3. The interaction between the enzymes and acceptor substrates shows non-Michaelian kinetics with respect to N-acetyl-D-glucosamine but normal behaviour towards N-acetyl-D-mannosamine and D-glucose. 4. Both N-acetyl-D-glucosamine and N-acetyl-D-mannosamine inhibit the phosphorylation of D-glucose; this inhibition appears to be mixed in character. 5. The facts that the enzymes catalyse the phosphorylation of N-acetyl-D-mannosamine and D-glucose do not detract from the designation of the enzymes as N-acetyl-D-glucosamine kinase. Phosphorylation of glucose in vivo by these kinases is unlikely.

    Title Castration Effects on the Response of Rat Pituitary Cells to Luteinizing Hormone-releasing Hormone: Retention in Dispersed Cell Culture.
    Date July 1980
    Journal Endocrinology
    Title Thyroid Hormones and the Precocious Induction of Hepatic Glucokinase in the Neonatal Rat.
    Date February 1980
    Journal European Journal of Biochemistry / Febs
    Excerpt

    1. Oral intubation of glucose is more effective than intraperitoneal injection in inducing the premature appearance of hepatic glucokinase in suckling rats. 2. The inducing effect of glucose is enhanced by treatment of the animals 12 h or more earlier with 1 microgram triiodothyronine/g body weight. 3. Low but significant activities of glucokinase appear at the normal time of development in hypothyroid neonatal rats. Intubation of glucose into 13-day-old and 24-day-old hypothyroid results in the rapid appearance of glucokinase similar to that in normal animals treated likewise. 4. The enhancing effect of thyroid hormones on glucokinase induction by glucose does not necessarily mean that the normal postnatal increase in plasma thyroid hormones is essential for the normal appearance of glucokinase activity at the time of weaning. Other possible explanations are discussed.

    Title Evaluation of in Vitro Incubation Systems for the Study of Gonadotropin Release.
    Date December 1979
    Journal Endocrine Research Communications
    Excerpt

    A detailed study was undertaken in order to determine if a pituitary-half incubation system were a suitable model for the study of anterior pituitary response to estradiol and LHRH. Considerable variation in the gonadotropin content of randomized pituitary halves was observed. Much less variation was found in matched halves. During the initial thirty minutes incubation of pituitary halves, a large spontaneous release of gonadotropins was observed. Time course secretion studies indicated that by four hours incubation, in the presence of 50 ng/ml LHRH, cumulative secretion of LH and FSH had far exceeded that of controls. Elevations in both cumulative secretion and rate of secretion were evident within 15-30 minutes of incubation. Regardless of LHRH dose, only 2-4% of either gonadotropin was secreted. Estradiol in the range of 10, 100, 500, 1,000 and 50,000 pg/ml had no significant effect on pituitary response to LHRH or on basal release, tissue levels or total gonadotropin. Based on these results, it was concluded that while the pituitary-half incubation system may be suitable for studying LHRH induced gonadotropin secretion, it is apparently of insufficient sensitivity to allow the collection of meaningful data concerning the effects of estradiol alone on gonadotropin secretion or estradiol modulation of LHRH induced gonadotropin secretion.

    Title Diagnostic Value of Brain Biopsy.
    Date February 1979
    Journal The Journal of Pediatrics
    Title Computer Assisted Tomography and Pneumoencephalography in Nontumorous Hydrocephalus in Infants and Children.
    Date September 1978
    Journal Journal of Computer Assisted Tomography
    Title Hypocycloid Tomography in Encephalography to Demonstrate Deep Hemorrhage of the Brain.
    Date July 1978
    Journal Acta Radiologica. Supplementum
    Excerpt

    Five cases of spontaneous deep brain hemorrhage have been presented. Two hemorrhages occurred above the tentorium and three below. The advantages of encephalography combined with tomography in diagnosing and following patients with deep brain hemorrhage are demonstrated in each case.

    Title Precocious Development of Rat Hepatic Glucokinase [proceedings].
    Date June 1978
    Journal Biochemical Society Transactions
    Title Transactional Analysis.
    Date March 1978
    Journal The Dental Assistant
    Title Ultrastructural and Physiological Studies of the Stalk-sectioned Rat Pituitary Gland.
    Date June 1977
    Journal The American Surgeon
    Excerpt

    A pituitary stalk-section rat model was prepared that would be suitable for the study of the direct effects of steroids on the pituitary gland. In this model, the hypophyseal stalk was severed surgically and metal foil was inserted to prevent regeneration of portal vessels. The major portion of the pituitary no longer had a direct communication with the hypothalamus, and the only blood supply was from short portal vessels that supply the dorsolateral part of the gland. The effectiveness of the lesion was demonstrated by very high levels of serum prolactin and very low FSH and LH. Light microscopic examination revealed a large infarct in the central region of the gland with stainable cells in the peripheral area. Fourteen days after stalk-section, the infarct had shrunken appreciably, and after 21 days it was replaced by a scar. Ultrastructural studies revealed the presence of functional mammotropes in the peripheral regions of the pituitary gland, while other cells were smaller, less active, and contained fewer secretory granules. After LHRH administration there was an increase in serum LH, thus indicating the presence of a substantial number of viable gonadotropes. This was confirmed by the ultrastructural findings which now showed the appearance of large, well-granulated gonadotropes. When LHRH was given to estradiol 17-beta primed ovariectomized stalk-sectioned rats, there was a great augmentation of LH release, and many castration cells were now apparent. Therefore, the administration of estrogen and LHRH was needed for the gonadotropes of the isolated pituitary to function optimally in the synthesis and secretion of FSH and LH. The stalk-sectioned animal model permits evaluation of pituitary function isolated from hypothalamic control while it leaves the surviving pituitary gland in its normal anatomical position.

    Title A Place for the Surgical Treatment of Epilepsy.
    Date June 1977
    Journal The American Surgeon
    Title Multiple Forms of Hepatic Glucokinase.
    Date May 1977
    Journal Biochemical Society Transactions
    Title The Purification in High Yield and Characterization of Rat Hepatic Glucokinase.
    Date August 1976
    Journal The Biochemical Journal
    Excerpt

    A new improved procedure for the purification of rat hepatic glucokinase (ATP-d-glucose 6-phosphotransferase, EC 2.7.1.2) is given. A key step is affinity chromatography on Sepharose-N-(6-aminohexanoyl)-2-amino-2-deoxy-d-glucopyranose. A homogeneous enzyme, specific activity 150 units/mg of protein, is obtained in about 40% yield. The molecular weight of the pure enzyme was determined by several procedures. In particular, sedimentation-equilibrium studies under a variety of conditions indicate a molecular weight of 48000 and no evidence for dimerization; reports in the literature of other values are discussed in the light of this evidence on the pure enzyme. The amino acid composition suggests that hepatic glucokinase is closely related to rat brain hexokinase and also the wheat "light" hexokinases.

    Title Potentiation of Luteinizing Hormone Release by Estradiol at the Level of the Pituitary.
    Date January 1976
    Journal Neuroendocrinology
    Excerpt

    The objective of this study was to determine the influence of estradiol-17beta and/or progesterone on gonadotropon secretion at the level of the pituitary. Female rats in which the hypothalamo-hypophyseal connections had been permanently interrupted after castration served as the experimental model in which the effect of estradiol and/or progesterone on LH-RH-induced gonadotropin release was examined. In out experimental animals, LH secretion was readily activated by LH-RH administration. LH release was greatly augmented by the prior administration of estradiol benzoate (1 mug/kg b.w./day). Progesterone (5 mg/day) in the absence of estradiol did not modify the 10-min responese to LH-RH but reduced the enhancement of LH secretion caused by estradiol pretreatment. Our findings suggest that estradiol potentiated the releasing effect of LH-RH at the level of the gonadotroph, whereas progesterone interfered with the potentiation effect. Plasma levels of FSH were not significantly elevated above the basal value by the administration of LH-RH alone, or in combination with estradiol and/or progesterone.

    Title Photosynthetic Nitrogen Fixation by Blue-green Algae.
    Date September 1975
    Journal Photophysiology
    Title Fsh and Lh Response in the Rat After Intravenous, Intracarotid or Subcutaneous Administration of Lh-rh.
    Date March 1975
    Journal Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (new York, N.y.)
    Title A Functional Isolated Pituitary Preparation.
    Date March 1975
    Journal Surgical Forum
    Title The Intrasellar Subarachnoid Recess. Some Clinical and Radiologic Observations.
    Date July 1973
    Journal Acta Radiologica: Diagnosis
    Title The Regulation of Rabbit Liver Fructose 1,6-diphosphatase Activity by Phospholipids in Vitro.
    Date May 1973
    Journal The Biochemical Journal
    Title Cranial Arteritis Associated with Herpes Zoster. Case Report with Angiographic Findings.
    Date April 1973
    Journal Radiology
    Title The Effect of an Iminostilbene Derivative (g32883) on Peripheral Nerves.
    Date April 1973
    Journal Journal of the Medical Association of Georgia
    Title Fistula Detection in Cerebrospinal Fluid Leakage.
    Date January 1973
    Journal Journal of Neurology, Neurosurgery, and Psychiatry
    Title Further Consideration of Sellar Changes Associated with Increased Intracranial Pressure.
    Date September 1972
    Journal The British Journal of Radiology
    Title Acute Meningeal Reaction Following Pantopaque Myelography.
    Date April 1971
    Journal Journal of Neurosurgery
    Title Structure, Physiology, and Biochemistry of the Chryophyceae.
    Date December 1969
    Journal Annual Review of Microbiology
    Title Electrophysicological Changes Associated with Experimental Diabetes.
    Date August 1968
    Journal Neurology
    Title Late Results of Cervical Discectomy and Interbody Fusion: Some Factors Influencing the Results.
    Date May 1968
    Journal The Journal of Bone and Joint Surgery. American Volume
    Title A Review of Current Concepts Regarding Intracranial Saccular Aneurysms. 3. Therapy.
    Date April 1968
    Journal Journal of the Medical Association of Georgia
    Title A Review of Current Concepts Regarding Intracranial Saccular Aneurysms.
    Date February 1968
    Journal Journal of the Medical Association of Georgia
    Title Comparative Studies of Experimental Blood Vessel Anastomosis Using Biological Adhesives.
    Date January 1968
    Journal Southern Medical Journal
    Title A Review of Current Concepts Regarding Intracranial Saccular Aneurysms. Ii. Symptoms, Signs and Diagnostic Procedures.
    Date January 1968
    Journal Journal of the Medical Association of Georgia
    Title The Value and Limitations of Brain Scanning: a Review of 401 Consecutive Cases.
    Date April 1967
    Journal Clinical Radiology
    Title Some Photochemical Properties of Chloroplast Preparations from the Chrysomonad Hymenomonas Sp.
    Date October 1966
    Journal Biochimica Et Biophysica Acta
    Title Analysis of User Behavior in Accessing Electronic Medical Record Systems in Emergency Departments.
    Date
    Journal Amia ... Annual Symposium Proceedings / Amia Symposium. Amia Symposium
    Excerpt

    Electronic patient tracking and records systems in emergency departments often connect to hospital information systems, ambulatory patient records and ancillary systems. The networked systems may not be fully interoperable and clinicians need to access data through different interfaces. This study was conducted to describe the interactive behavior of clinicians working with partially interoperable clinical information systems. We performed 78 hours of observation at two emergency departments, shadowing five physicians, ten nurses and four administrative staff. Actions related to viewing or recording data in any system or on paper were recorded. Collected data were compared along clinical roles and contrasted with findings across the two hospital sites. The findings suggest that differences in the levels of interoperability may affect the ways physicians and nurses interact with the systems. When tradeoffs in functionality are necessary for connecting ancillary systems, the effects on clinicians and staff need to be considered.

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